Blood-vessel-on-chip-pack

Reproduce physiological flow conditions for vasculature assays

Reproduce physiological shear stress

Control the flow rate and profile with accuracy

Undirectional flow

Mimic what actually happens inside blood vessels

Use the device of your preference

Circular tubules, monolayers, barrier models can be easily connected

Image credit: Choroidal vessels in the human eye with red blood cells. Peter Maloca. Attribution 4.0 International (CC BY 4.0).

Blood-vessel-on-chip setup

Microfluidics has gained relevance in cell biology due to its ability to replicate a more physiologically relevant microenvironment for cell and tissue assays. In this light, one of the microenvironments where flow is more prominent is inside blood vessels. Cells on the walls of blood vessels are subjected to elevated shear stress due to the blood flow rates.

Our blood-vessel-on-chip pack is designed to replicate the relevant flow rates found inside blood vessels with the flexibility of using the chip of your preference so you can better reproduce the tissue microenvironment of your interest. A pressure-driven flow controller, combined with a flow sensor, can provide a range of flow rates (from 0.5µl to 5ml/min) and flow profiles (linear, pulsatile, stepwise, etc.) to mimic the pathophysiological conditions found in the vasculature.

The recirculation bridge is a set of arranged valves that allows the recycling of the media, enabling its enrichment with metabolites, the preservation of conditioned media containing bioactive molecules, and the economy of large volumes of media in long-term experiments.

The level sensors, integrated into the smart culture tube rack, automate the experiment setup and changes between reservoirs. Also, in case of leakage, they stop the flow before the reservoirs become empty and air can reach the cells.
check valves recirculation bridge setup

The blood-vessel-on-chip pack includes:

Flow sensor (Galileo, MIC)

Recirculation bridge

Smart culture tube rack

Software (Galileo user interface)

Reservoirs for precursor solutiona

Several falcon reservoirs

Tubings and fittings

Microfluidic chip of your choice

User guide

Compatibility of blood-vessel-on-chip pack

Our blood-vessel-on-chip Pack was designed to be compatible with commercially available or homemade chips (specific adaptors might be required), the most common microscopy, and supernatant assays. If you work with a particular chip or assay not listed below, please get in touch with us!

Chips

biosensor chip test
PMMA-device-station

PMMA Device Station

From mold development to high-resolution PMMA chip fabrication

✓ Fast process

✓ Multiple applications

✓ Simple and innovative setup

PMMA

Synvivo

And many more! 

Applications

Assays
syringe pump application chemical development

TEER

Immunostaining of endothelial cell markers

Albumin reabsorption

3D reconstructions

Perfusion assay, using fluorescent dyes

Pharmacokinetics assays

Immunohistochemical and histopathological characterization

Toxicity assays

And many more! 

  1. Effects of shear stress and flow profile on endothelial cells
  2. Migration and evasion/invasion of circulating tumor cells
  3. Barrier structure and permeability
  4. Vascularisation
  5. Angiogenesis

If you want to know more about organ-on-a-chip technology, have a look at this review!

Customize your pack

We offer compatible instruments for standard commercialized chips from various brands. We can customize your pack to suit your specific needs with guidance from our specialists, who will suggest the best instruments and accessories. We’ll also help you set up the perfusion platform.

Frequently asked questions

Can I order a pack?

Since Packs are products that are still being developed, we have a few eligibility criteria to maximize their success rate. A discussion with our experts is needed to determine your specific needs to offer you a personalized response.

The components of the setup can be sterilized. Our user guide provides detailed information on how to do it.

Yes! Our experts will establish which instruments are best suited for your application, such as the type of flow sensor or the number of flow controller channels you need to perform your experiment. Contact us using the “talk to our experts” green button above.

Our instruments are in beta testing phase and can be tested as a pack or individually, so get in contact with our team to know how our beta testing program works.

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Funding and Support

The ALTERNATIVE and LIFESAVER projects helped develop this instrument pack. 
These projects are funded by the European Union’s H2020-LC-GD-2020-3, grant agreements No. 101037090 (ALTERNATIVE) and 101036702 (LIFESAVER).

Products & Associated Accessories

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