Mini Pump for Cell Culture
Easy cell culture media recirculation inside the incubator
Incubator-friendly
Place everything neatly inside the incubator
Autonomous
Control everything with the built-in screen
Easily used with large volume reservoirs
Use the reservoir of your choice
Mini pump for cell culture
The mini pump was designed to facilitate the recirculation of media for long-term cell culture inside the CO2 incubator.
Its simple design, with only one inlet, one outlet, and a screen that controls everything through a handle, takes care of all the fluidics and allows you to focus on biology. It is also easily adapted to different reservoir volumes, from Eppendorfs to 1 L bottles.
The mini pump can be used in two main configurations.
First, as a closed-loop using only one reservoir as inlet and outlet of media.

The second configuration comprises two reservoirs and can be used as a reservoir-to-waste system, meaning there is no media recirculation. One reservoir provides fresh media, and the second collects used media. The cells only receive fresh input.
It is also possible to turn this configuration into a recirculation setup by adding level sensors that automatically switch between reservoirs.


U251-MG (glioblastoma cells) cultured for 7 days inside the CO₂ incubator with the mini pump at 3 µl/min. The cells were cultured inside a microfluidic straight-channel chip, connected to a reservoir with fresh medium on the inlet and a waste container on the outlet, as illustrated in the schematics above.
Applications and compatibility
The mini pump is compatible with organ-on-a-chip applications and is not limited by your chip design or manufacturer.
Applications can include:

Stem Cell Culture
Automated and fail-safe long-term microfluidic cell culture system.
✓ Highly controlled microenvironment
✓ Fail-safe mechanism
✓ Automated sequences
Stem Cell Culture

Blood-brain Barrier on Chip
Plug-and-play instrument pack for long term BBB on a chip study
✓ Relevant microenvironment
✓ Automatized organ-on-chip perfusion
✓ Plug-and-play microfluidic platform
Blood-brain Barrier on Chip

Drug Screening
Organ-on-a-chip technology emerged in the early 21st century as a revolutionary approach to simulate human organ functions in vitro. So, what is an organ-on-a-chip? What are its main applications and advantages? And to what extent is it a promising tool for drug testing and disease modeling? We answer all these questions in the following review.
Drug Screening

Sheer Stress
Cell culture system for shear-sensitive cell lines
✓ Safe Neuron Culture Under Flow
✓ Highly Controlled Microenvironment
✓ Up to 3-week Long Cell Cultures
Sheer Stress

Blood-Vessel-on-Chip Pack
Reproduce physiological flow conditions for vasculature assays
✓ Reproduce Physiological Shear Stress
✓ Unindirectional Flow
✓ Use the Device of Your Preference
Blood Vessel Formation & Occlusion (Atherosclerosis)

Bone-on-a-Chip Pack
Easily mimic the complex bone physiology and extracellular environment
✓ Control Complex Microenvironments
✓ Up to 3-week Long Cell Cultures
✓ Fail-Safe Mechanism
✓ Plug-and-Play Platform
Bone Homeostasis and Disease (Osteoporosis)
Immune Response
Models of Physiology and Disease
And many more!
Chips

PMMA Device Station
From mold development to high-resolution PMMA chip fabrication
✓ Fast process
✓ Multiple applications
✓ Simple and innovative setup
PMMA
Synvivo
Ibidi
Microfluidic ChipShop
And many more!
Reservoirs
The mini pump can be connected to any reservoir, from Eppendorf to large bottles, in fully closed or open systems.

Mini pump technical specifications
The technical specifications of the mini pump can be found below. Don’t hesitate to contact us using the green button above.
Components | Technical Specifications |
---|---|
Flow Rates | down to 1 µl/min |
Wetted material | Polyphenylsulfone (PPSU) or stainless steel |
Operating Temperature | 5-50 °C |
Maximum pressure | up to 500 mbar |

Customize your pack
Our instruments can be added to different setups depending on your specific needs. In this light, our microfluidic specialists will advise you on the best instruments and accessories depending on your needs and will accompany you during the system’s setup.
Frequently asked questions
Can the mini pump be placed inside the CO2 incubator?
Yes, the mini pump was designed to be used inside the CO2 incubator. It can also be used on the bench without problems or near a microscope.
Is the mini pump powered by battery?
No, the mini pump is connected to a power outlet through a cable.
Do I need a computer to operate the mini pump?
No, the mini pump is autonomous. You can control all its functions through the built-in screen.