Mini Pump for Cell Culture

Easy cell culture media recirculation inside the incubator

Incubator-friendly

Place everything neatly inside the incubator

Autonomous

Control everything with the built-in screen

Easily used with large volume reservoirs

Use the reservoir of your choice

Mini pump for cell culture

The mini pump was designed to facilitate the recirculation of media for long-term cell culture inside the CO2 incubator.

 

Its simple design, with only one inlet, one outlet, and a screen that controls everything through a handle, takes care of all the fluidics and allows you to focus on biology. It is also easily adapted to different reservoir volumes, from Eppendorfs to 1 L bottles.

 

The mini pump can be used in two main configurations.

 

First, as a closed-loop using only one reservoir as inlet and outlet of media.

mini-pump_schematics1

The second configuration comprises two reservoirs and can be used as a reservoir-to-waste system, meaning there is no media recirculation. One reservoir provides fresh media, and the second collects used media. The cells only receive fresh input.

 

It is also possible to turn this configuration into a recirculation setup by adding level sensors that automatically switch between reservoirs.

mini-pump_schematics2
mini pump for cell culture experiments simple perfusion for 7 days

U251-MG (glioblastoma cells) cultured for 7 days inside the CO₂ incubator with the mini pump at 3 µl/min. The cells were cultured inside a microfluidic straight-channel chip, connected to a reservoir with fresh medium on the inlet and a waste container on the outlet, as illustrated in the schematics above.

Applications and compatibility

The mini pump is compatible with organ-on-a-chip applications and is not limited by your chip design or manufacturer.

Applications can include:

stem-cell-on-matrix

Stem Cell Culture

Automated and fail-safe long-term microfluidic cell culture system. 

✓ Highly controlled microenvironment

✓ Fail-safe mechanism

✓ Automated sequences

Stem Cell Culture

Blood-brain barrier on a chip mouse cappillaries

Blood-brain Barrier on Chip

Plug-and-play instrument pack for long term BBB on a chip study

✓ Relevant microenvironment

✓ Automatized organ-on-chip perfusion

✓ Plug-and-play microfluidic platform

Blood-brain Barrier on Chip

mouse model Organ-on-chip models

Drug Screening

Organ-on-a-chip technology emerged in the early 21st century as a revolutionary approach to simulate human organ functions in vitro. So, what is an organ-on-a-chip? What are its main applications and advantages? And to what extent is it a promising tool for drug testing and disease modeling? We answer all these questions in the following review. 

Drug Screening

neuron culture bioreactor

Sheer Stress

Cell culture system for shear-sensitive cell lines

✓ Safe Neuron Culture Under Flow

✓ Highly Controlled Microenvironment

✓ Up to 3-week Long Cell Cultures

Sheer Stress

blood vessel

Blood-Vessel-on-Chip Pack

Reproduce physiological flow conditions for vasculature assays

✓ Reproduce Physiological Shear Stress

✓ Unindirectional Flow

✓ Use the Device of Your Preference

Blood Vessel Formation & Occlusion (Atherosclerosis)

bone osteoporosis wellcome collection

Bone-on-a-Chip Pack

Easily mimic the complex bone physiology and extracellular environment

✓ Control Complex Microenvironments

✓ Up to 3-week Long Cell Cultures

✓ Fail-Safe Mechanism

✓ Plug-and-Play Platform

Bone Homeostasis and Disease (Osteoporosis)

Immune Response

Models of Physiology and Disease

And many more! 

Chips

PMMA-device-station

PMMA Device Station

From mold development to high-resolution PMMA chip fabrication

✓ Fast process

✓ Multiple applications

✓ Simple and innovative setup

PMMA

Synvivo

Ibidi

Microfluidic ChipShop

And many more! 

Reservoirs

The mini pump can be connected to any reservoir, from Eppendorf to large bottles, in fully closed or open systems.

microfluidic_reservoirs

Mini pump technical specifications

The technical specifications of the mini pump can be found below. Don’t hesitate to contact us using the green button above.

 

ComponentsTechnical Specifications
Flow Ratesdown to 1 µl/min
Wetted materialPolyphenylsulfone (PPSU) or stainless steel
Operating Temperature5-50 °C
Maximum pressureup to 500 mbar
microfluidic-cell-culture-system-converter
Pressure splitter, to be kept outside the incubator (left); and cell culture platform (right), which can be entirely placed inside the incubator.

Customize your pack

Our instruments are compatible with standard commercialized chips from different brands.

Our instruments can be added to different setups depending on your specific needs. In this light, our microfluidic specialists will advise you on the best instruments and accessories depending on your needs and will accompany you during the system’s setup.

Frequently asked questions

Can the mini pump be placed inside the CO2 incubator?

Yes, the mini pump was designed to be used inside the CO2 incubator. It can also be used on the bench without problems or near a microscope.

No, the mini pump is connected to a power outlet through a cable.

No, the mini pump is autonomous. You can control all its functions through the built-in screen.

eu_funded_en
biopros european project logo microfluidics innovation center

Funding and Support

The BioProS project helped develop this instrument. This project has received funding from the European Union’s Horizon research and innovation program under HORIZON-CL4-2021-DIGITAL-EMERGING-01-27, grant agreement no. 101070120 (BioProS).

Products & Associated Accessories

Main-Logo-MIC-PURPLE

Talk to
our experts