Giant Unilamellar Vesicle (GUV) Production Pack
Plug-and-play pack for monodisperse giant unilamellar vesicle production
Monodisperse and unilamellar guvs
Bottom-up approach for bigger than 1 µm biomimetic structures
Automated high-throughput production
Produce giant unilamellar vesicles with custom sequences
Gret monodispersity and reproducibility
Better control than electroformation or other GUV synthesising methods
Cell-sized GUV picture adapted from Ho et al. (2016), double emulsion production with oleic acid by Teh, Shia-Yen, et al. (2011)
GUVs production pack setup
Microfluidics allows better reproducibility, monodispersity, vesicle size control, encapsulation efficiency, and membrane homogeneity, making it the most efficient method to create GUVs compared to bulk techniques.
This Pack comprises an flow controller that controls three channels through flow sensors. One channel has the inner aqueous solution, the other the oil/lipid-based solution, and the third the outer aqueous solution and the solutions meet at the junctions of the double emulsions chip, which can be homemade or commercial, such as the microfluidic ChipShop fluidic 1480.
This pack can be customized to fit any double emulsion application; contact us for personalized advice and expertise.
The GUVs production pack contains:
Flow sensor (Galileo, MIC)
Software (Galileo user interface)
Flow controller
Fittings, tubings & luers
Reservoirs
User guides for instruments
Can contain the fluidic 1480 microfluidic chip from microfluidic chipshop if needed
Compatibility of the pack
Applications
GUVs produced by this pack can be used for:
Membrane-protein interaction assays
Drug delivery studies
Artificial cell-like systems
Encapsulation assays
Membrane deformation studies
and much more!
Analysis
Depending on your application, your results can be gathered by:
Bright-field microscopy
Fluorescent microscopy (if stained accordingly)
Secretion assays
and much more!
Customize your pack
Our Packs can be modified depending on your specific needs. For example, this pack can include a sequential injection function to change the composition of the membrane easily during giant unilamellar vesicle production. Our microfluidic specialists will help you choose the best instruments and accessories depending on your needs and will accompany you during the setup of the microfluidic platform.
