Endothelial cell image from Freya Mowat (wellcome collection – CC BY 4.0), Channel drawing figure by Raasch et al. (2015), Vascular cells drawing figure by van der Meer et al. (2009).
ENDOTHELIAL CELL CULTURE PACK
Unbox, set up and start your experiments right away
Controlled shear stress with laminar flow for medium distribution
Culture conditions closer to the in vivo cell layer conditions
Microfluidics endothelial cell culture
Endothelial cell layer culture in a microfluidic channel is now accessible using this pack for an in vitro model as close as possible to in vivo conditions. Our plug-and-play instruments are the easiest solution to transition from classical 2D cell culture in wells to organ-on-chip technologies.
Based on the high-accuracy OB1 flow controller (Elveflow) and a membrane biochip, this solution contains all the required microfluidic parts for researchers to set up their endothelial cell culture with improved EC marker protein expression and good cell adhesion. Our experts will also accompany you during the setup of the microfluidic platform.
Experiments involving organs-on-chips are complex systems to set up. Fortunately, we were engaged in several organ-on-chip projects over the years and gathered valuable knowledge in this research field. We are always eager to discuss ideas with researchers!
A basic microfluidic endothelial cell layer culture pack contains a pumping channel coupled with a distributor to seed two different kinds of cells on both sides of the membrane in the chip. This allows the creation of a more physiologically relevant endothelial cell layer that can be used for new therapeutic developments or toxicity screening.
The distributor quickly injects different substances, like FITC-dextran, and a 3/2 valve is used to choose which channel to perfuse. The efficiency of the perfusion will be directly linked to the flow rate inside the upper and lower channels. Flow rates can be measured with a microfluidic flow sensor (e.g., Galileo).
This setup allows for the control of strain, shear stress, and pressure to approach physiologically realistic values. Thus, this pack’s experiment conditions are more relevant than those of classical static cell culture.
Microfluidic endothelial cell culture setup
An all-in-one pack guarantees perfect compatibility between the different parts, allows a quick experiment start, is piloted by a single software, and can be used for other different applications. This is why a pack is the easiest way to set up your microfluidic experiment.
The endothelial cell culture pack contains:
- OB1 MK4 flow controller (Elveflow)
- Rotative valve (MUX Distributor) (Elveflow)
- A MUX recirculation that creates a recirculation of the medium
- Microfluidic flow sensor (eg. Galileo)
- Cross-flow membrane chip from microfluidic ChipShop (Suggestion: Fluidic 568)
- Several falcon reservoirs for the sample and the medium
- 3/2 Microfluidic valves
- A MUX wire to control the valves
- Pressure splitter manifold
- Tubings and connectors
- Software (Galileo user interface)
- Primary human umbilical vein endothelial cells (HUVECs) (if necessary)
We have recently published a review comparing different perfusion recirculation systems.
Why use microfluidics for endothelial cell culture?
Firstly, using microfluidics is a way to decrease the amount of potentially valuable samples needed for a reaction. Moreover, fluid properties can be precisely tuned to mimic in vivo conditions at the microfluidic scale as much as possible.
Thus, creating microfluidics models of human organs is more effective than 2D classical or animal models because microfluidic systems allow more physiologically accurate conditions. Furthermore, the European Union and the public are pushing to reduce the use of animal models.
Microfluidic endothelial cell layers allow more flexible, precise, and efficient experiments to assess drug toxicity or pathogen effects on endothelial cells.
References
1. A. D. Van der Meer, A. A. Poot, M. H. G. Duits, J. Feijen, I. Vermes, “Microfluidic Technology in Vascular Research”, BioMed Research International, vol. 2009, Article ID 823148, 10 pages, 2009.
Microfluidics endothelial cell culture principle
Much work has investigated how endothelial cells react to various chemical, biological, or physical stimuli in vitro, as vascular dysfunction is a critical consequence of significant diseases like diabetes or cancer [1]. Organs-on-chip is an up-and-coming field that will prevent drug clinical failures and replace classical 2D cell culture and animal model testing [2].
Endothelial cell culture models can be created in microfluidic channels separated by a membrane to obtain physiologically relevant biomechanical conditions with realistic flow, strain, shear stress, and pressure [3-4].
For example, Raasch et al. compared the viability and morphology of endothelial cell cultures in traditional static and dynamic conditions, with flow cells, and in a microfluidic system, as illustrated below.
The low flow conditions were 0.7 dyn cm−2 with only minimal flow-related mechanical stimulation and high shear stress with ten dyn cm−2, which corresponds to the human in vivo situation in larger arteries and venules and represents the mean shear stress rate of the common human carotid artery.
The authors have not found a significant difference in cell viability between low-flow conditions. However, cells presented better elongation and density in the high-flow condition when inside the microfluidic chip, indicating better adaptability [5].
This kind of microfluidic system has also been used to study the endothelial cells at the blood-brain barrier to make a human-relevant disease model [6]. The permeability of the endothelial cell layer in function of the shear stress applied on the layer has also been studied in a two channels system separated by a membrane [7].
Check out our other organ-on-a-chip packs: blood-brain barrier on-a-chip, gut-on-a-chip, bone on-a-chip and lung-on-a-chip.
References
- A. D. van der Meer, A. A. Poot, M. H. G. Duits, J. Feijen, I. Vermes, “Microfluidic Technology in Vascular Research”, BioMed Research International, vol. 2009, Article ID 823148, 10 pages, 2009
- Capulli A. K., Tian K., Mehandru N., Bukhta A., Choudhury S.F., Suchyta M., Parker K.K., Approaching the in vitro clinical trial: engineering organs on chips, Lab Chip, 2014,14, 3181-3186
- Estrada R., Giridharan G.A., Nguyen M-D, Roussel T-J, Shakeri M, Parichehreh V., Prabhu S.D., and Sethu P., Endothelial Cell Culture Model for Replication of Physiological Profiles of Pressure, Flow, Stretch, and Shear Stress in Vitro, Anal. Chem. 2011, 83, 8, 3170–3177
- Estrada R., Giridharan G.A., Nguyen M-D, Roussel T-J, Prabhu S.D., and Sethu P., Microfluidic endothelial cell culture model to replicate disturbed flow conditions seen in atherosclerosis susceptible regions, Biomicrofluidics, 5, 032006 (2011)
- Martin Raasch et al. Microfluidically supported biochip design for culture of endothelial cell layers with improved perfusion conditions, 2015, Biofabrication 7 015013
L. M. Griep, F. Wolbers, B. de Wagenaar, P. M. ter Braak, B. B. Weksler, I. A. Romero P. O. Couraud, I. Vermes & A. D. van der Meer, A. van den Berg, BBB ON CHIP: microfluidic platform to mechanically and biochemically modulate blood-brain barrier function, Biomedical Microdevices volume 15, 145–150 (2013)
Young E. W. K., Watson M. W. L., Srigunapalan S., Wheeler A. R., Simmons C. A., Technique for Real-Time Measurements of Endothelial Permeability in a Microfluidic Membrane Chip Using Laser-Induced Fluorescence Detection, Anal. Chem. 2010, 82, 808–816
Customize your microfluidic endothelial cell culture
The cross-flow membrane chip included in this pack can be in Cyclo Olefin Copolymer (COP) or Polystyrene (PS), hydrophilized or not. Depending on your application, two different pore sizes can be chosen: 0.2 µm or 8 µm. Custom-specific membranes can also be made on demand.
This pack can be significantly personalized depending on your requirements.
Bubbles can be a problem for cell culture. Thus, we can provide a bubble remover to tackle this issue.
Other packs for organ-on-chips are available. Check out the blood-brain barrier on-a-chip, gut-on-a-chip, bone on-a-chip and lung-on-a-chip.
You can contact our scientists to answer any questions about this endothelial cell layer culture pack and how it can match your applications.
– Check our other Packs for various applications –
Can I order a pack?
A Pack can be available under certain conditions. Since Packs are products that are still being developed, we have a few eligibility criteria to maximize their success rate. A discussion with our experts is needed to determine your specific needs to offer you a personalized response.
How can we help in your experiment?
The Packs applications are still under development, so we are not able to give you the tips or troubleshooting advice that we usually give in our user guides for the possible challenges you could face for your specific experiment. That said, we can always guarantee reliable and high-precision microfluidic flow control. Our microfluidic instruments are high-performance, versatile and user-friendly. Our experts will bring support and expertise during the setup and implementation of the pack.
Can a pack be customized based on my specific application?
Yes! Our experts will establish which instruments are best suited for your application, such as the type of flow sensor or the number of flow controller channels you need to perform your experiment. Contact us using the “talk to our experts” green button above.
Can I buy individual instruments?
You can order our instruments on the product section of our website.
