Image Credit: Villi in the small intestine, Paul Appleton, University of Dundee. Licence: Attribution 4.0 International (CC BY 4.0)
Inflammatory Bowel Disease Model
Automatically collect time-dependent samples of your IBD model
Relevant and controlled microenvironment under flow
Collect volumes from microliters to milliliters
IBD pack setup
The inflammatory bowel disease model comprises a fluidic controlling unit (OB1 pressure controller, flow sensors, and valves) before the chip that allows high-precision liquid handling. Thus, your cells receive a continuous supply of media and nutrients. At the same time, the addition of immune-relevant cytokine triggers for IBD can be done at the most convenient time through an easily controlled set of valves.
Then, the cytokine release profile over time can be assessed due to a fraction collector (MUX distributor) connected to the chip outlet. Samples can be automatically collected according to your requirements of time and volume.
The IBD pack typically contains:
- OB1 flow controller (Elveflow).
- Flow sensor (MFS or BFS, Elveflow).
- One or more rotative distribution valves, according to the application (MUX distributor, Elveflow).
- 3/2 Valves and controller (MUX wire, Elveflow).
- Microfluidic chip.
- Falcon reservoirs, connectors, tubing, filters…
- ESI SOFTWARE (Elveflow) (optional: SDK libraries; C++, Python, MATLAB, LabVIEW).
Check our fraction collector page for more details.
Compatibility of IBD model
Our inflammatory bowel disease model pack was designed to be compatible with commercially available or homemade chips (specific adaptors might be required) and the most common supernatant assays. If you have a particular chip or assay that is not listed below, please contact us!
Chips
Homemade devices, PDMS, PMMA, etc.- Ibidi
- Microfluidic ChipShop
- Netri
- Synvivo
- and more!
Assays
- ELISA
- Western Blot
- SEC
- HPLC
- Mass Spectrometry
- and more!
Customize your pack
Our packs are fully customizable to fit your needs perfectly. Our specialists and researchers will help you choose the best instruments and accessories and accompany you during the setup of the microfluidic platform until you obtain your first experimental results.
Contact our experts to answer any questions about this fraction collector pack and how it can match your specifications!
– Check our other Packs for various applications –
Is the setup sterilizable?
Yes, we have developed a simple protocol for sterilization and cleaning that is provided along with the user guide.
Can the setup be placed inside the CO2 incubator?
The chip with the cells can be kept inside the incubator and the flow sensors as well, after treatment upon demand. The electronics, such as the pressure controller and the collection valves, should be kept outside the CO2 incubator.
Can a pack be customized based on my specific application?
Yes! Our experts will establish which instruments are best suited for your application, such as the type of flow sensor or the number of flow controller channels you need to perform your experiment. Send us a message at: innovation@microfluidic.fr
Can I buy individual instruments?
You can order the instruments on the product section of our website.
Funding and Support
The results of the LOCAi, NeuroTrans, and Enhpathy project helped develop this pack. The LOCAi project has received funding from the French National Research Agency (ANR) and the German Federal Ministry of Education and Research (BMBF) in the frame of the 1st German-French Joint Call for Proposals on Artificial Intelligence. The European Union’s Horizon 2020 MSCA-ITN has funded the Enhpathy and NeuroTrans projects under grant agreements No. 860002 (ENHPATHY project) and 860954 (NeuroTrans project).
