CELL PERFUSION WITH OXYGEN CONTROL
Pressurize your media with the desired oxygen concentration
Compact and portable pump
Stable flow in the physiological profiles of your choice
Oxygen control setup
We have assembled all the components to perform successful perfusion with continuous O2 control. The setup displayed below is meant to continuously and stably perfuse media in a highly controlled microenvironment: a pre-mixed gas bottle is plugged into the cell culture pump, pressurizing the reservoirs with the correct mix of gasses for your experiment; temperature is kept by a stage top incubator that allows for long-term live cell imaging with ease.
The cell culture oxygen pack contains:
- Cell culture pump
- Microfluidic flow sensor (eg. Galileo)
- Fittings, tubings, luers
- Reservoirs
- Microfluidic chip (application-dependent; suggestion microfluidic Chipshop fluidic 480)
- Stage top incubator
- Software (Galileo user interface)
- User guides for instruments
This instrument can also be adapted to our automated cell culture platform.
For applications requiring fluid injection to test the effects of drugs, for example, a rotary valve or a check valve recirculation bridge can be added to the setup. Check out our automated recirculation system and this application note that explains how to assemble the recirculation bridge.
Oxygen levels in dynamic cell culture
Adequate microenvironment parameters are crucial for a thriving cell culture. Oxygen in the cell culture is one of the parameters that must be surveyed to ensure healthy cell growth and division.
However, it is rarely monitored in traditional cell culture flasks since it is assumed that the oxygen from the atmosphere will diffuse into the media at sufficient rates.
On the other hand, dynamic cell cultures are closed-loop systems in which the changes in concentration of essential parameters, such as oxygen, are more pronounced due to the minute volumes. Therefore, they require closer monitoring and surveillance.
Applications
- Red blood cell studies
- Oxygen supply for gas impermeable chips
- Effects of oxygen concentration on cell viability and gene expression
- and many more!
Chip compatibilities
- Microfluidic ChipShop
- Homemade devices, PDMS, PMMA, etc.
- Ibidi
- Synvivo
- and more!
Oxygen control pack specifications
The Oxygen control pack contains a set of instruments for continuous cell culture perfusion and monitoring. The cell culture pump controls the fluid flow.
The table summarizes the main specifications of the Oxygen control system.
Component |
Technical specifications |
| Cell culture pump | 4 Channels (0 to 2000 mbar) |
| Microfluidic flow sensor (eg. Galileo) | Range from 0.5 to 10,000 µL/min |
| Cell culturing chamber | Microfluidic chips (2.55 x 7.55 cm) |
| Stage top incubator | 0 to 70oC; k-frame bottom |
Customize your pack
Our instruments can be added to highly customizable setups, so you can take advantage of the extra flexibility to adapt them depending on your specific needs. Our microfluidic specialists will advise you on the best instruments and accessories depending on your needs and will accompany you during the setup of the microfluidic platform.
Our instruments are compatible with standard commercialized chips from different brands.
– Check our other Packs for various applications –
Is the setup sterilizable?
The reservoirs and tubings are disposable but can be sanitized with a cleaning protocol. The flow sensors can also be sanitized with the same protocol. Connectors can be autoclaved.
Can I do anaerobic/hypoxic experiments with this setup?
For example, you can plug a nitrogen bottle into the cell culture pump to pressurize a deoxygenated media. However, the gas permeability of the tubing and the gas tightness of the fluidic system connections must be considered to ensure no oxygen permeates into the system during the experiment.
Can the pump be placed in the CO2 incubator?
The gas control pump should be kept outside the CO2 incubator. The flow sensors can be placed inside the incubator.
Can it measure the oxygen concentration in the media?
Currently, the pump can only control the gas composition based on the pre-mixed gas input supplied. It is advisable to add oxygen sensors to the microfluidic circuit or chip if there’s a need to measure the precise concentration of oxygen.
Funding and Support
LIFESAVER and Tumor-LN-oC projects helped develop this pack. These projects are funded by the European Union’s H2020-LC-GD-2020-3 and H2020-NMBP-TR-IND-2020, grant agreements No. 101036702 (LIFESAVER), and No. 953234 (Tumor-LN-oC).
