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Image credit: Proteus, SEM. David Gregory & Debbie Marshall.

MICROBIOLOGY INCUBATOR

Follow your bacteria's growth and responses in real time under the microscope
FOLLOW YOUR BACTERIA IN REAL TIME

Small and mobile incubator chamber for the microscope stage

ACCURATE TEMPERATURE CONTROL

Keep your bacteria at 37.5°C ± 0.5°C outside the incubator

UP TO 2 SIMULTANEOUS CULTURES

Test different conditions in the same system

How to culture bacteria under the microscope?

The current advances in analytical assays allow greater detail and information out of experiments. For example, it is possible in microbiology to follow single-cell gene expression through time [1] or follow the growth rate and gene expression of several generations of bacteria [2] in microfluidic devices.

 

However, to do so also requires better ways of gathering data. For example, single-cell monitoring can be significantly improved if the bacterial culture is maintained on top of the microscope stage. But that, in turn, requires minimal culturing conditions outside the incubator.

 

The microbiology incubator was designed to address precisely this need. As shown below, it keeps an ideal temperature under the microscope for better imaging data gathering for either static or dynamic flow conditions.

References

1. Young, J., Locke, J., Altinok, A. et al. Measuring single-cell gene expression dynamics in bacteria using fluorescence time-lapse microscopy. Nat Protoc 7, 80–88 (2012). https://doi.org/10.1038/nprot.2011.432

2.Microfluidic chemostat for measuring single cell dynamics in bacteria. Lab Chip, 2013,13, 947-954

Microbiology incubator setup

A pressure-driven flow controller and a microfluidic flow sensor (eg. Galileocan control the flow in experiments with bacteria in dynamic conditions. 

 

This setup can also be combined with other packs, such as media perfusion and recirculationsequential injection, bubble trapping, cell-cell interaction monitoring, or precision sampling for more complex applications.

stage top incubator schematics

The Microbiology incubator pack contains:

  • Flow controller
  • Microbiology incubator (in development)
  • Microfluidic flow sensor (eg. Galileo)  
  • Several Eppendorfor falcon reservoirs
  • Tubings and luers
  • Microfluidic chips if needed
  • Software (Galileo user interface)
  • User guide

 

 

For more information on the chamber, check our stage top incubator page!

The performance of the microbiology incubator

Bacillus subtilis is a gram-positive soil bacteria related to many critical applications in the food industry and environmental sciences. Given its wide range of utility and that it is not a pathogen to humans, it is a widespread model organism for bacterial adaptability, development, and biofilm formation.

 

It is found in diverse environments, from soil to human gut microbiome, with radical differences in temperature. In addition, they produce endospores that can withstand extreme temperatures. Therefore, depending on the behavior to be studied, high temperatures, constant fluctuations, or stable 37oC can be of interest as culturing conditions. 

Here, the goal was to assess the performance of the microbiology incubator in comparison with a traditional incubator at 37oC and cultures grown at room temperature.

 

The microbiology incubator was initially designed to house dynamic cultures, such as cultures grown in chips subjected to media flow. However, it can easily accommodate static cultures and different culturing devices, such as the Eppendorf demonstrated below.

Methods

Freshly inoculated Lysogeny Broth (LB) medium was distributed in 18 Eppendorfs that were divided in three groups and placed in a standard incubator at 37 °C, in the microscope stage incubator at 37 °C and left outside at room temperature.

microbiology incubator setup2
Eppendorf icon by Anthony Ledoux. The Noun project.

Results

Preliminary results of bacterial growth in the microbiology incubator show equivalent curves when compared to a standard incubator.

microbiology incubator results
Optical density measurements of Bacillus subtilis cultures over time.

Each group’s turbidity of one Eppendorf was measured every hour for 6 hours with a spectrophotometer at 600 nm wavelength (OD600). OD600 measurements are routinely used to measure microbial proliferation in liquid cultures. Bacillus subtili’s ideal culture temperature is 37 °C. 

Therefore, faster growth is expected in inoculates stabilized at such temperatures. Both incubator and microbiology incubator showed similar OD readings at each timestep, with both groups entering the exponential growth phase as opposed to the room temperature group.

microscope stage incubator results
(Left) Bacillus subtilis sample after incubation at 37 °C during 6 hours imaged at 40x through the microbiology chamber and the glass window of a PDMS microfluidic chip. Single bacteria can be observed without any distortion from the chamber thermal glass and focused through different planes. (Right) Turbidity differences between the Eppendorfs keep at 37 °C (left) and room temperature (right) after 6 hours can be seen with naked eye.

Customize your microbiology incubator pack

In the past years, several commercialized and laboratory-made microfluidic chips have been developed and tested to perform dynamic cell and bacteria culture efficiently. We can include one of them with different surface modifications inside the pack. A chip can also have several separate channels.

 

Our products and packs are fully customizable to fit your needs. Our microfluidic specialists and researchers will help you choose the best instruments and accessories and accompany you during the setup of the microfluidic platform until you can obtain your first experimental results.

 

Contact our experts for any questions about this microbiology incubator and how it can match your specifications!

 

– Check our other Packs for various applications –

Is the microbiology incubator gas-tight

No, the chamber allows gas exchange with the atmosphere.

Yes, we have developed a simple protocol for sterilization and cleaning that is provided along with the user guide.

No, the reservoirs can be kept at room temperature. The microbiology incubator was designed to warm the liquids to the right temperature before reaching the bacteria.

Packs like this one are available under certain conditions. Since Packs are still under development, we have a few eligibility criteria to maximize their success rate. Discussions with our experts are needed to determine your specific needs and offer you a personalized response. Contact us using the “talk to our experts” green button above.

Funding and Support

The ActiveMatter, ACDC, and Protomet projects helped develop this instrument pack, with funding from 

the European Union’s Horizon 2020 MSCA-ITN under grant agreement No 812780 (Active Matter),

the European Union’s Horizon 2020 research and innovation program under grant agreement No 824060 (ACDC project),

and the European Union’s Horizon 2020 MSCA-ITN under grant agreement No 813873 (ProtoMet).

 

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