Track immediate cellular responses to calcium changes
Introduce reagents in a controlled sequence
Maintain cell viability with gentle handling
How our solution enhances calcium imaging
In live-cell calcium imaging experiments, timing is crucial when introducing different solutions to observe intracellular calcium flux. Manual switching introduces inconsistencies that can distort results and compromise data accuracy.
Our microfluidic-based automated perfusion system eliminates this problem by delivering precisely controlled calcium influx and efflux cycles.
Researchers can study real-time calcium signaling in immune cells, neurons, and cardiac tissues with improved accuracy. The system is also ideal for high-throughput screenings of calcium channel blockers and other drugs, facilitating drug discovery and pharmacological research.
In addition, it maintains controlled microenvironments for long-term imaging experiments, ensuring optimal cell viability. By eliminating timing discrepancies, this system enhances reproducibility across experiments, leading to more consistent and reliable data.
This system also prevents sample contamination and minimizes reagent waste, making it a cost-effective and high-precision solution for laboratories working on calcium imaging.
Perfusion pump technical specifications for Calcium imaging
Pressure control | |
| Pressure range | -400 to 600 mbar |
| Pressure stability | 0.2 mbar |
| Air flow rate | 0.1L/min at atmospheric pressure Possibility to work with higher air flow rates by reducing the pressure range |
Flow control | |
| Microfluidic flow sensor | Monitoring and feedback loop flow control available |
| Flow rates | From 0.1 µL/min to 5 mL/min |
Stage top Incubator specifications for Calcium imaging
| Characteristics | Specifications |
| Dimensions (mm) | 30.5 x 130 x 168 (h x w x l) |
| Base K- Frame | 3.5 x 110 x 160 (h x w x l) |
| Dimensions of internal usable space | 25 x 89 x 130 (h x w x l) |
| Dimensions of the bottom glass (ITO glass) | 1) 72 x 110 with a thickness of 1.1 mm 2) 50 x 25 with a thickness of 0.6mm 3) 50 x 22 with a thickness of 0.12 mm |
| Temperature range | Room Temperature to 70 oC |
| Temperature accuracy | ± 0,5 oC |
Other applications
Our automated perfusion system is not limited to calcium imaging- it can also be used in:
- Electrophysiology studies for ion channel research
- Neuroscience experiments requiring controlled neurotransmitter applications
- Cardiovascular research for studying calcium-driven cardiac contractions
- T-cell activation and immune response studies in microfluidic environments
- And many more!
Can the solution exchange timing be adjusted?
Yes, the system allows full customization of perfusion timing to match your experimental protocol.
Is this system compatible with existing microscopes?
The stage top incubator fits a k-frame microscope.
Can it be used for drug testing?
Absolutely. The system is ideal for high-throughput screening of calcium-modulating compounds.
Funding and Support
This project has received funding from the European Union under HORIZON-EIC-2022-PATHFINDEROPEN-01-01, grant agreement No. 101099719 (THOR).
