FRACTION COLLECTOR FOR CELL CULTURE
Automatically collect samples in a defined period
Automatically collect multiple data points during dynamic cell culture
Collect samples down to 150 microliters
Fraction collector pack
Automated fraction collector for improved results
Manual sampling is time-consuming and prone to heterogeneity due to inherent human variability during the collection process. This heterogeneity can negatively affect the reproducibility of the results, jeopardizing the research’s perceived value [1].
Also, human interaction during sampling usually requires the experiment to be halted and can inadvertently introduce contamination [2].
A fraction collector designed for cell perfusion
The advantages of dynamic cell culture regarding improved physiological relevance are becoming ever more established [3].
To profit from dynamic cell culture’s advantages, the sample collection must be adapted to the continuous media flow without disrupting cell culture. The Fraction Collector Pack was designed to do precisely that.
Collect samples adapted to your application
Sample collection has to match the analysis to be performed afterward. Some assays, such as Elisa and mass spectroscopy, require minute volumes, going as low as 100 µl.
A fraction collection system must enable sample volumes that align with these requirements.
The Fraction Collector Pack allows the collection of volumes down to 100 µl and can collect these volumes at defined periods with an easy and user-friendly automation setup.
References
1. Csete, Marie. “Q&A: What can microfluidics do for stem-cell research?.” Journal of Biology 9.1 (2010): 1.
2. Daniszewski, Maciej, et al. “Automated cell culture systems and their applications to human pluripotent stem cell studies.” SLAS TECHNOLOGY: Translating Life Sciences Innovation 23.4 (2018): 315-325.
3. Mehling, Matthias, and Savaş Tay. “Microfluidic cell culture.” Current opinion in Biotechnology 25 (2014): 95-102.
Fraction collector setup
The fraction collector pack comprises state-of-the-art instruments for precise fluid control and sample collection automation. These instruments are all controlled by the ESI software that allows simple automation of each device, so they work together according to your needs. The pack can be used with the chip design of your preference.
The fraction collector pack typically contains:
- OB1 flow controller (Elveflow)
- Flow sensor (MFS or BFS, Elveflow)
- A rotative distribution valve (MUX Distributor, Elveflow)
- Microfluidic chip
- Falcon reservoirs, connectors, tubing, filters,…
- ESI Software (optional: SDK libraries; C++, Python, MATLAB, LabVIEW)
Fraction collector principle
Several applications can benefit from automated fraction collection. Here, the synergistic effect of two drugs is explored as an example. A µ-Slide III 3D Perfusion from Ibidi is used to culture cells.
Channel A is a negative control in which only the medium is perfused.
Channel B explores the synergistic effect of Drug 1 and Drug 2, with an initial exposure to Drug 1 followed by the concurrent perfusion of Drugs 1 and 2.
Channel C is a positive control, with the perfusion of only Drug 2. Each channel is connected to a rotary valve programmed to automatically collect samples of 150 µl every two hours. The samples are then analyzed for the temporal secretion of specific molecules linked to the action of the drugs on the cells.
Automated fraction collection applications
- Temporal release of cytokines by brain cells.
- Release of extracellular vesicles.
- Molecular released during inflammatory responses.
- Uptake and metabolism of substances of interest.
Customize your pack
Our instruments and packs are fully customizable to fit your needs perfectly. Our specialists and researchers will help you choose the best instruments and accessories and accompany you during the setup of the microfluidic platform until you can obtain your first experimental results.
We can provide reservoirs, flow sensors, bubble detectors, traps, or any relevant microfluidic instruments.
Contact our experts to answer any questions about this fraction collector pack and how it can match your specifications!
– Check our other Packs for various applications –
Is the setup sterilizable?
Yes, we have developed a simple protocol for sterilization and cleaning that is provided along with the user guide.
Can the instrument be customized based on my specific application?
Yes! Our experts will establish which instruments are best suited for your application, such as the type of flow sensor or the number of flow controller channels you need to perform your experiment. Drop us a line at innovation@microfluidic.fr
Can I buy individual instruments?
You can order our instruments on the product section of our website.
Funding and Support
The results of the LOCAi, NeuroTrans, and Enhpathy project helped develop this instrument pack. The LOCAi project has received funding from the French National Research Agency (ANR) and the German Federal Ministry of Education and Research (BMBF) in the frame of the 1st German-French Joint Call for Proposals on Artificial Intelligence. The European Union’s Horizon 2020 MSCA-ITN has funded the Enhpathy and NeuroTrans projects under grant agreements No. 860002 (ENHPATHY project) and 860954 (NeuroTrans project).
