MFMET II aims to strengthen the metrological foundations needed for reliable standardization in microfluidics and organ-on-chip technologies.

A central objective is to define standardized measurement procedures for core microfluidic quantities that drive performance and comparability, including particle-laden flows (droplets, bubbles, particles, cells), shear stress, pressure drop, flow resistivity, and device volumes (dead and total volume). This helps move the field away from “device-specific” validation toward methods that are repeatable and comparable across platforms.

In parallel, the project will develop protocols for integrating sensors, actuators, and fluidic components using scalable and sustainable manufacturing approaches, while addressing sterilization, contamination prevention, and material-medium interactions, such as absorption/adsorption, coatings, and wettability changes, which are especially critical for organ-on-chip systems.

Finally, MFMET II will establish guidelines for quality control and reliability (including test approaches, failure modes, and safety aspects). The project will also develop an integrated microfluidic setup with multiple sensors and actuators intended to act as a metrological transfer standard. The project’s outcomes will be disseminated through publications, protocols, white papers, and recommendations aimed at regulators, standardization bodies, and the global microfluidic community.