Patented Technology

Slides for Immunohistochemistry Protocol

Micropatterned slides for high-resolution Immunohistochemistry and confocal microscopy

Multiplex staining ready

Supports multi-target Immunohistochemistry (IHC) protocols for detecting multiple biomarkers

No more air bubbles

Micropatterns automatically remove air bubbles

Compatible with heat & enzyme treatments

Withstands high temperatures and enzymatic digestion for antigen retrieval

Optimized microscope slides for high-quality immunohistochemistry

An improperly placed coverslip over a fixed tissue sample can introduce unwanted air pockets, significantly affecting the quality of your immunohistochemistry protocol and potentially compromising days of work.

These trapped air pockets can hinder antibody penetration, disrupt the even distribution of reagents, and degrade image clarity in both immunohistochemistry and confocal microscopy, leading to unreliable results. Additionally, air pockets can cause inconsistent staining intensity, making quantitative analysis of biomarkers difficult and affecting diagnostic accuracy.

To overcome these challenges, we developed a micropatterned sample holder specifically designed to prevent air entrapment. This advanced slide features engraved micropatterns or grooves strategically placed around the sample visualization area. Acting as capillary barriers, these structures help retain liquid within the designated area, ensuring consistent and uniform staining for immunohistochemistry applications.

Additionally, capillary forces naturally hold the coverslip in place, eliminating the need for external sealants like wax or paraffin. This not only simplifies sample preparation but also preserves tissue morphology and enhances antibody interaction, leading to more precise and reproducible staining.

Moreover, the glass slide’s surface and micropattern design can be tailored to accommodate various sample types, making it an ideal solution for both standard and advanced Immunohistochemistry protocols.

References

Image credit: Detecting cancer in human tissues, LM. Aamir Ahmed, Jane Pendjiky and Michael Millar / Wellcome Collection

Compatibility and applications

Some other biological applications also include:

Histopathology

Clinical diagnostic

Immunofluorescence

Drug Screening

And many more!

Slides for immunohistochemistry protocols

The technical specifications of the product ensure air bubble-free imaging while maintaining high-quality staining and sample integrity for immunohistochemistry protocols.

	Technical Specifications
Wetted materials	Glass or thermoplastics (PVD, CVD, PECVD)
Dimensions	Micropatterns: Depth: 100 µm Width: 100 µm Distance between micropatterns: 100 µm
Working volumes	TBD

Frequently asked questions

Can the working volume of the microscope slides be altered for different Immunohistochemistry protocols?

Yes, our slides are designed to accommodate different tissue section sizes and staining volumes. Contact our team for more information.

Do they work with any type of coverslip?

Yes, currently our slides are made on demand, so we can adjust the features to any coverslip dimensions. In any case, they are compatible with standard coverslips.

Can the shape of the micropatterns and observation chamber be altered?

Yes, contact our team for more information on custom designs.